C. Elegans imaging with SCAPE 2.0
The nematode Caenorhabditis elegans is an attractive model organism for neuroscientific research. Its stereotypical nervous system consists of only 302 neurons in the hermaphrodites, with neural connectivity fully mapped. The transparency of C. elegans, its small size and its relatively simple nervous system make it ideal for imaging the entire nervous system in restricted or mobile conditions. Of the 302 identifiable neurons, most are tightly packed in the head and tail ganglia. The small size of the neurons and the high oscillation speed of the head (> 100 µm in less than 1 second) require an imaging system with high spatial and temporal resolution. We demonstrate full body dual color GCaMP / RFP recording with single cell resolution at> 25 volumes per second. Compared to confocal spinning disk systems, SCAPE offers a significantly higher temporal resolution and less photobleaching, which is required for downstream cell tracking or long-term recording.
For more information, see our new SCAPE 2.0 paper:
Voleti V, Patel KB, Li W, Perez Campos C, Bharadwaj S, Yu H, Ford C, Casper MJ, Yan RW, Liang W, Wen C, Kimura KD, Targoff KL, Hillman EMC. Real-time volumetric microscopy of in vivo dynamics and large-scale samples with SCAPE 2.0. Nature methods. 16 (10): 1054-62. (2019)